News Researchers Develop First Accurate Method to Assess Important Protein in MS, Other Diseases Researchers Develop First Accurate Method to Assess Important Protein in MS, Other Diseases by Patricia Silva, PhD | March 24, 2015 Share this article: Share article via email Copy article link Researchers at the University of Copenhagen and Rigshospitalet in Denmark have for the first time developed an accurate method to measure apolipoprotein M (apoM), a protein thatĀ is involved in several diseases like diabetes but also arteriosclerosis and sclerosis, disorders characterized by the stiffening of structures usually by replacement of the normal tissue by connective tissue. The study is entitled āProtein unfolding allows use of commercial antibodies in an apolipoprotein M sandwich ELISAā and was recently published in the Journal of Lipid Research. The protein apoM circulates in the plasma attached to high-density lipoprotein (HDL) particles. ApoM is important for cholesterol metabolism and has been recently shown to transport the signaling lipid sphingosine-1-phosphate (S1P), which has been implicated in various inflammatory diseases such as rheumatoid arthritis and multiple sclerosis. āWe know that apoM is of importance to the development of arteriosclerosis and cardiovascular diseases. Previously, we have discovered that apoM carries a small fatty molecule, S1P, which plays a part in both diabetes and sclerosis. Whether or not apoM is of importance, remains to be seen, however, our new and secure measuring methods open up completely new perspectives on the research being conducted in these areasā said the studyās senior author Dr. Christina Christoffersen in a news release. To establish an accurate, reliable and efficient method to measure apoM has been a challenge for researchers worldwide. āThe protein is attached to the good cholesterol, HDL, which makes measuring it difficult. It is folded like a small funnel, but expressed in popular terms, we have managed to unfold it, which makes it much easier to identifyā explained Dr. Christoffersen. [adrotate group=”4″] Danish researchers have developed a method that specifically recognizes the human apoM in plasma using commercially available apoM antibodies and a āsandwichā ELISA-based assay (an enzyme-linked immunosorbent assay to detect sample antigens through antibodies). The antibodies were carefully selected and the method was found to have a high degree of specificity, sensitivity and precision. Although it took several years to reach this technique, it is relatively simple and can be easily performed by researchers worldwide. āThese antibodies are actually commercially available, so all you have to do is order them and start measuringā concluded Dr. Christoffersen. The research team believes this method can be implemented in every laboratory and that the ability to accurately measure apoM will allow further studies focused on its biological functions and eventual clinical implications in several diseases such as multiple sclerosis. Print This Page About the Author Patricia Silva, PhD PatrĆcia holds a PhD in medical microbiology and infectious diseases from the Leiden University Medical Center, Netherlands, and completed a postdoctoral research fellowship at the Instituto de Medicina Molecular, Lisbon, Portugal. Her work in academia was mainly focused on molecular biology and the genetic traits of infectious agents such as viruses and parasites. PatrĆcia earned several travel awards to present her work at international scientific meetings. She is a published author of several peer-reviewed science articles. Tags antibodies
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